Part:BBa_K1864001:Experience

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Applications of BBa_K1864001

We built yeast which contains two parts. The first part is CSBV-RdRP (Part:BBa_K1864000) and the second part is T7 RNA polymerase. Meanwhile, IPTG could induce the operation of T7 RNA polymerase. Therefore,we tested the induction results of different IPTG concentration. And we got the data which

User Reviews

UNIQ4bf022a4c3bacdb4-partinfo-00000000-QINU UNIQ4bf022a4c3bacdb4-partinfo-00000001-QINU

IPTG inductive effect

We tested the IPTG inductive effect in T7 RNA polymerase. In our project,we built the yeast which contained two parts to produce dsRNA. Meanwhile,IPTG could induce the operation of T7 RNA polymerase.Therefore,we could test the concentration of dsRNA to estimate the inductive effect.

The inductive effect of different concentration IPTG

We tested dsRNA concentration with the induction of 0.1,0.2,0.3,0.4,0.5,0.6 mmol/L IPTG after 4 hours

The inductive effect of different time length

We tested dsRNA concentration with the induction time length from 0 to 9 hours. And we tested three group which involved concentration.